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DNAse only works in the presence of Mg++. We use DNAse I, 300 mcg/ml,
(60,000 Dornase U/mg) to break all sorts of cell clumps. I can't
remember it failing (but this may be a reflection on the biology of my
memory, as much as the biology of cell clumps).
Calman
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From: JOY MUNDY
Sent: Thursday, July 10, 1997 12:49 PM
To: cyto-inbox
Subject: Lymph Nodes that Clump
Hi
Can anybody help me with a cell suspension solution that will inhibit
cell clumping. We disaggregate lymph node tissue for routine flow but
have heaps of trouble with cell clumping. We have tried Mg and Ca
free
RPMI, also DPBS with DNAse to no avail. We keep blocking up the probe
of the XL which is a bit of a bother.
Willing to try anything!!!!!!!
Joy Mundy
Dept. Human Immunolgy
Institute of Medical & Veterinary Science
Adelaide, South Australia 5000
Ph 61-8-82287476; Fax 61-8-82324092
jmundy@immuno.imvs.sa.gov.au
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web