Re: conjugation vs. sandwich
ClaudeC81@aol.com
Sun, 30 Mar 1997 22:22:08 -0500 (EST)
There seem to be many responses to this query, I thought I'ld add some
support for biotinylation of the antibody. It's as easy as conjugating to
FITC, brighter (because it is a sandwich, and in many cases is brighter even
than an immunoglobulin sandwich ... I'ld like to hear if you disagree...),
and gets around the problem of "bad" IgG (not-so specific, esp. for B cells).
But if you really want to go with a secondary immunoglobulin conjugate, go
with an Fab (no cross-linking) or Fab'2 conjugate (test it first). Just
tossing this out in hopes it helps, but an ulterior motive is personal
edification, so I'm open to "clarifications". Claude.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
