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I'll pop across and discuss this with you later, but in the mean time other
people may have some experience of this so I'm sending it to the list.
Apparently DTT (DiThioThreitol) is used in some flow techniques to reduce
proteins in situ leaving cells intact. It would be interesting to see if
anyone has any comments on this, and suggest what concentrations to use to
prevent cell lysis.
Ray
lAt 9:21 am +0200 3/6/97, Michel Canton wrote:
>Hi, Andy:
>In regards to your question about cell membrane protein, I can advise you to
>contact Dr Philippe Poncelet at BioCytex in France (Tel: +33 (0) 4 91 94 29
>39 or Fax: +33 (0) 4 91 47 24 71). He is certainly a great expert in this
>field. Unfortunately he does not have E-mail.
>Have great success in your projects.
>Michel Canton
Ray Hicks
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