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>>>It would appear the 'Calibur is able to directly detect APC, Cy5 or Cy7
>>>as the 4th color. In that case the use of ANY of the PE tandems may
>>>present some problems with the second laser. Of course this isn't a
>>>problem if you use PerCP for your "near red" (FL3).
I don't think the 635 nm laser will effectively excite Cy7 directly, and even if
it does the stock 661/16 in FL4 will not pass it's emission. We are certainly
able to detect APC and Cy5 in the FL4 channel.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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