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Use calibrated beads: add a known concentration of beads to all samples and
calculate the ratio of WBC:Beads. (BDIS has a product called TrueCOUNT - tubes
with beads.
Dennis
*************************************************************************
* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
* http://www-core.ucsd.edu/flow-core.html *
*************************************************************************
______________________________ Reply Separator _________________________________
Subject: Re: Absolute counts from flow cytometry
Author: douglas@HELIX.NIH.GOV at @UCSD
Date: 11/15/96 8:38 AM
At 04:29 PM 11/14/96 EST, you wrote:
>
>We are trying to generate absolute counts for different cell types in the
>peripheral blood of mice. What are your preferred methods? ......
Jeff Carrell
Human Genome Sciences
Jeff,
You may want to look at LDS-751. It is a nucleic acid dye not requiring
permeabilization and can be used in whole blood with no lysing.
See the following reference:
Multidimensional Flow Cytometric Blood Cell Differentiation without
erythrocyte lysing, Terstappen, et al. in Blood Cells (1991) 17:585-602.
The dye is available from Exciton, Inc (513) 258-3937, Dayton, Ohio.
Good luck.
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>Date: Fri, 15 Nov 1996 08:38:36 -0400
>To: Cytometry Mailing L
>From: Doug Terle <douglas@HELIX.NIH.GOV>
>Subject: Re: Absolute counts from flow cytometry
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
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Phone: (765)-494-0757;
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