Re: PE/Cy5 on a FACS Calibur
steven micko (steven_micko@email.eushc.ORG)
Wed, 25 Sep 96 11:03:06 EST
Calman,
We are a beta site for the second (red) laser on a FACSCalibur. I do my
daily QC with Calibrites (FTC, PE, blanks, PerCP and APC), and it works
fine. Our biological work uses PerCP, APC, and Cychrome, and I have had
no complaints. I don't know about PE/Cy5; is it the same as CyChrome?
The present status of 4 color work on the Calibur is that it is in Beta
testing. We use cross-beam compensationS between FL1 & FL2, FL2 & FL3,
FL3 & FL4.
Steve Micko
Emory Hospital
Atlanta
______________________________ Reply Separator _________________________________
An associate has asked me a third hand question regarding the utility of
using PE/Cy5 conjugates on a FACScaliber in 3 color applications.
Questions for the group and for BD lurkers in particular:
1. Do PE/Cy5 conjugates work any better or worse on a Calibur than on a
FACSCAN in 3 color/ 1 laser applications.
2. What is the present status of 4 color work on the Calibur? Any plans
for cross-beam compensation to allow simultaneous use of FITC, PE,
PE/Cy5 and APC?
Thanks,
Calman Prussin
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
