Re: Cy3
Howard Shapiro (hms@shapirolab.com)
Wed, 2 Jul 1997 22:50:27 -0400 (EDT)
Cy3 can be excited at 488 nm, but not very efficiently; its excitation
maximum is at about 540 nm, and its emission maximum is at about 565 nm.
You could use a flow cytometer with any of the following light sources; the
closer to 540 nm, the better:
Argon ion laser; 515 or 528 nm (528 needs special optics)
Krypton or mixed gas ion laser; 520 or 530 nm
Any big system may have these (e.g., FACS Vantage, MoFlo, EPICS
Frequency-doubled YAG laser; 532 nm
Green He-Ne laser; 543 nm
The green He-Ne's have often been installed on Coulter Elites; the B-D
FACScount also has a green He-Ne but its software (CD4 counts only) is
probably too inflexible to make this an option.
Mercury arc lamp; 546 nm
These can be found on B-D's old FACS Analyzer, Partec's instruments, and
the Bio-Rad Bryte (or the old Skatron).
All the sources mentioned above have been used at one time or another on
Cytomutts as well...but it doesn't pay to build or buy an instrument or
maybe even a laser for one antibody. Why does this person need to use Cy3?
-Howard
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
