acrylamide and monoclonal antibodies

Byrne, Fergus, Ph.D. (fbyrne@med1.medsch.ucla.edu)
Fri, 21 Mar 97 10:46:00 PST

you can find a way to do this in the big green hardback book by harlow ad
Lane called "Antibodies, a loaboratory manual " .. Nearly all vaguely immnuo
labs. have a copy of it - it's like maniatis. Can't remember exactly where
but it is definetly in there.

Fergus

UCLA

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To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
From: Pete Macardle <Peter.Macardle@cc.flinders.edu.au>
Subject: acrylamide and monoclonal antibodies
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Dear all,
I remember seeing a technique for making monoclonal antibodies by injecting
the mouse with a band of protein cut from an acrylamide gel. I cannot find
the reference on Medline. Is anyone familiar with this method? In particular
I want to know how much acrylamide a mouse can have before toxic effects
overcome it.

Thanks in advance.
Pete

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