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The PerCP and PE-Cy5 will work on the FACScan very well. Both have the
emission maxima around 675nm (677 for PerCP and very close for PE-Cy5, but I
do not remember exact value). You only have to know what kind of a filter do
you have for the collection of the FL3 signals. 650LP as far as I remember
was the standard in FACScan. It will work all right. As you probably know
PE-Cy5 travels under so many aliases one may easily get confused. DAKO,
Serotec, Immunotech etc call it simply PE-Cy5, PharMingen calls it
'Cy-Chrome', Caltag prefears 'TriColor', Sigma for a change likes 'Quantum
Red', and I suspect Exalpha's 'AlphaRed' is the one too.
As far as I know there is no commercial source of anti-human or anti-mouse
immunoglobulins directly conjugated to PE-Cy5 or PerCP. There are ways to
bypass it, though.
1. Get the biotinylated antibody and the streptavidin conjugated PerCP from
Becton Dickinson, or streptavidin-PE-Cy5 from, say DAKO. The best source of
biotinylated goat anti -human/-mouse MoAbs is Jackson ImmunoResearch.
2.Get those anti-immunoglobulin MoAbs conjugated to FITC or PE and the other
ones you want to use (in a 3 color setup) conjugated to PerCP or PE-Cy5.
This idea does not work if you need ALL of your MoAbs (FL1,2,3) directed
against immunoglobulins, of course.
Hope this helps a bit,
Janek
--------------------------
mmbrozek@cyf-kr.edu.pl
http://www.cyf-kr.edu.pl/~mmbrozek
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web