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Instead of multidrop sorts you might want to try reducing the droplet
formation frequency a little and doing single drop sorts. We have done
similar sorts onto filters using 25 kHz droplet generation frequency and
single droplet sorts. We get a much tighter droplet trajectory with this
setup. This works especially well if your sorter allows you to cells based
on the phase relationship of their arrival with the sort charge pulse (i.e.
require that the cell be in the center 60% of the sort pulse).
gary
Vincent Falco laments:
>
>
> My problem is this:During some sorts I get ,what I will call a spurious extra
> drop. To explain more,there are times I sort to polycarb filters.When all is
> well I get a single spot which contains the desired cell population.When all
> is supposed not well I get my desired single spot with cells and just about a
> quarter of inch from the desired spot I observe a spurious spot,which does
> not contain the desired cells.
Gary Durack
University of Illinois Biotechnology Center
voice (217) 244-0559 fax (217) 244-0466
durack@uiuc.edu
http://www.life.uiuc.edu/biotech
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web