 |
 |
 |
 |
 |
I have used both commercial hemaline and water as sheath
in the FACSCalibur and FACScan with no problems - sometimes
I like to clear the lines and the droplet containment motor of salts
and run with something a lot cheaper - however some samples
are uniquely susceptible to small changes in tonicity as occurs when
the drop left behind in the sip tube between samples enters a subsequent
sample during sample change. Our Red cell samples for example
had to be run exclusively in hemaline and for DNA analysis you may
wish to use isoton(hemaline) exclusively because of the noted susceptibility
of PI staining to small tonicity differences. For the majority of
immunophenotyping
however, I would think no problems.
Gene Pizzo/UCONN Health
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web