 |
 |
 |
 |
 |
Acridine Orange is probably the fastest and the easiest method to
>evaluate apoptosis and/or cell cycle but ..... how can I be sure that it
>wouldn't contaminate our flow-cytometer?
On our Elite, we routinely change the sample tubing between applications
where a dye is known to remain in the tubing. You'll have to check how
feasible this is for your machine, but is a simple way to avoid
contamination of subsequent samples.
--------------------------------------------------------
Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
2/5/97 1:12:01 PM
E-mail: thomasm@nshs.edu
--------------------------------------------------------
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web