bone marrow -clinical question
Dr Cesar Nunez (cnunez@fs1.scg.man.ac.uk)
Wed, 21 May 1997 17:09:19 +0100
Dear Adrian:
We agree with Pete Macardle that rib specimens from cardiac surgery (and
sometimes from kidney DONOR transplant) are very good for normal bone
marrow studies. Also, it is possible to work with bone marrow derived from
head femur obtained during ortopedic surgery (hip replacement). The latest
one is more complicated and time consuming than flushing media through
the rib but at the end we have to work with what is available in house.
Anyhow, rib is better.
Also, you can freeze the remaining of the sample in case that
you want to analize another antigens. Although later studies are more
complicated because of apoptotic cells (neutrophils and monocytes).
It is important to have i) a good blocking system to avoid unspecific
antibody binding, ii) separation of apoptotic cells based on FSC and SSC,
iii) plus the antibody coktail suggested by Brad Sherburne (that looks
fine). Lymphocytes and blast usually remains unchanged.
We hope that this comments can help you to find a way to analyze this
difficult issue.
Please, do not forget. Good luck is also very important!
Cesar Nunez, MD.
University of Manchester
School of Biological Sciences
G.38 Stopford Building
Oxford Road
Manchester M13 9PT
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