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The general problem with bioassays of this type, whatever the indicator
parameter, is that one has to make sure that the observed change is due to
the drug in question, and not to something else in the test sample or to
some physiologic change in the cells. This necessitates careful design of
controls. I'd guess that this might be even harder for an assay based on
levels of surface markers than for one based on something like calcium flux
or membrane potential or on a percentage of cells undergoing a more-or-less
all-or-none change like membrane lysis or apoptosis.
-Howard Shapiro
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web