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Why not trying annexin V labeling instead of the TUNEL assay. It is easy,
does not need cell fixation and can be easily combined with surface labeling.
For details about the method read the article by van Engeland et al.,
Cytometry 24, 131-139, 1996.
Good luck,
Bert Schutte
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web