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------- Forwarded Message Follows -------
From: Dorothy Reimer <reimed@genemedicine.com>
To: cyto-inbox
Date sent: Wed, 23 Jul 1997 17:49:35 -0500
Subject: some flow questions
Dear Terry:
I am interested in following some internal fluorescence through tumour
cells during gene transfection procedures. I have been able to follow it
by flow to some extent, but I find that there is some autofluorescence
associated with these cells (directly dispersed from tumours which were
injected with some formulations). I have some information to believe that
trypan blue can be used to decrease to autofluorescence thereby enhancing
the signal to noise ratio. I have tried this with my cells, but fail to
see a shift to the left indicating a decrease in autofluorescence. I have
tried several different concentrations of TB in addition to several other
things. But, after talking with Mark, I am wondering about the mechanics
of how TB works. Do you as an experienced flow person have any suggestions
or comments to me about the use of TB as an inhibitor for autofluorescence.
Or is there something else that I could try. I really need to find a way
to enhance the separation on flow, since the population of cells that I
have that are positive are potentially quite low.
I would sure appreciate any ideas you can pass my way.
Thanks in advance for your help.
Dody Reimer, Senior Scientist, Gene Medicine.
Dr. Terry Hoy
Principal Research Officer
Department of Haematology Telephone 44 1222 743458
University of Wales College of Medicine FAX 44 1222 744655
Heath Park E-mail hoy@cardiff.ac.uk
CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
U.K.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web