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The following message is posted for Virginia Cox <cox@medicine.tamu.edu>.
Please reply to CYTOMETRY or to her address with a response.
>I have been trying to sort E. coli with the GFP gene inserted for a
>client. When we run the sorted bacteria through the machine to check for
>purity, we see little fluorescence & the light scatter has changed. We
>think we may be damaging the cells. Any experience or suggestions would
>be appreciated.
>
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Posted by
>
__________________________________________________________________
J.Paul Robinson, Ph.D., Professor of Immunopharmacology
Director, Purdue University Cytometry Laboratories
Purdue University Phone:(765)-4940757 FAX: (765)-4040517
EMAIL: robinson@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web