Nigericin contamination?
Wayne Green (Wayne.Green@genetics.utah.edu)
Thu, 10 Jul 1997 11:42:11 -0600
We have been running some pH studies using SNARF-1 on myocytes. The
investigator wants to use Nigericin to calibrate the pH measurments but we have
some concern about contaminating the sample lines and flow cell in the flow
cytometer. A very recent paper by P.H. Richmond & R.D. Vaughan-Jones (Pflugers
Arch - Eur J Physiol, No. 254, 1997- I have only provisional page numbers)
indicates that residual nigericin can strongly affect subsequent nigericin-free
samples in a superfusion flow chamber. They developed several extensive
cleaning procedures to remove the contaminating nigericin from their apparatus.
I have searched through the flow literature and have found references in
which nigericin is mentioned as a means of calibrating measurements, but have
found nothing addressing contamination of the instrument or cleaning nigericin
from tubing/flow cells. Does anyone have experience with this compound and is
contamination a problem in a flow cytometric application?
--
Wayne Green
wgreen@genetics.utah.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
