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does anybody know a source for fluorescent beads with changing fluorescence
properties (such as excitation of emission spectrum) with the pH value. I
want to measure the pH value of the lysosomes of phagocyting macrophages. The
beads should have about 2 um diameter. I have beads which show variations,
but they are really not monodispers, the fluorescence distribution is
extremely broad. I would like to have a sharper distribution. All laser
wavelength from UV to HeNe could be used.
Thank you in advance
Best regards to all flow people
Wolfgang Beisker
Dr. Wolfgang Beisker
GSF - Durchflusszytometrie phone: (+49)(0)89 3187 2842
Ingolstaedter Landstr. 1 FAX : (+49)(0)89 3187 3349
85764 Neuherberg EMAIL: Beisker@gsf.de
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web