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Hi-
I hope this is the proper route to post something on the archive. I've
tracked down several protocols for propidium staining, but I do have one
question they do not address. Don't you need to centrifuge the stained
samples before running them through the flow cytometer? I would
imagine that you still have some PI in solution, and that it would give
you an awfully high background signal. Any comments?
Thanks. This is a wonderful forum.
Bob Crow
crow@cbnmr.tamu.edu
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posted by
J.Paul Robinson, Purdue University Cytometry Labs
Professor of Immunopharmacology
robinson@flowcyt.cyto.purdue.edu PH:765-494 6449 FAX:765-494 0517
web http://www.cyto.purdue.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web