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We experienced a very unusual phenomenon as follows;
A few days ago, a healthy donor was performed the peripheral blood
progenitor cell collection on COBE Spectra following the G-CSF
mobilization protocol for allogeneic stem cell transplantation to
his brother suffering from an acute leukemia.
Because one of two HLA haplotypes was not totally mismatched, the
physician decided to select the CD34 positive stem cells and to reduce
the T lymphocytes using CEPRATE system. After the procedure,
CD34 positive cell count and lymphocyte subset tests were requested.
Everthing had been fine as we expected. However, the proportion of
CD19 positive lymphocytes in the final product was unbelievable.
IT WAS OVER 90 PERCENT OF TOTAL LYMPHOCYTES.
Even more, CD3 positive lymphocytes also expressed CD19.
The lymphocyte subset profile of pre-CD34 selection procedure
(PBPC product) was similar to that of normal peripheral blood and
CD3 positive lymphocytes did not express CD19.
We
thought that it might be an error but the exact same phenomenon
occurred on the next day.
Here is the illustration of our results.
CD19 | ... | . CD3- CD19+ 92%
| ..... | ... CD3+ CD19+ 7%
| ... | . CD3- CD19- 1%
| | CD3+ CD19- 0%
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CD4
Is there anyone who experienced similar phenomenon as ours?
And Is there anyone who has been studied the lymphocyte subsets
after CD34 selection?
Please give me any comments!
With Best Regards
===================================
Chang-Seok Ki, MD
Department of Clinical Pathology
Samsung Medical Center
50 Ilwon-Dong, Kangnam-Gu
Seoul, 135-710
Korea
Voice (82)-2-3410-2708
Fax (82)-2-3410-2719
E-mail kcdol@samsung.co.kr
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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