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The expert on sperm sorting is Larry Johnson at the U. S. Department of
Agriculture in Beltsville, which is a short drive away from your facility.
He and most other people who have successfully separated X and Y sperm have
relied on detection of differences in DNA content, using modified commercial
flow cytometers and staining cells with Hoechst 33342. Surface markers
evaluated to date have apparently not been as effective.
-Howard
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web