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In my laboratory work, I attempt to double stain the cells with Brdu (for
proliferation assay) and a cell surface marker. The cells I am working
with are the cell mixture of intestine epithelial cells and T cells. I
don't know if anybody has tried to do the similar things. It seems a
little hard to do since the anti-Brdu Ab has to enter the nucleus while
the other Ab has to bind to the cell surface moleculels. I would
appreciate any input. My email address is wangx01@doc.mssm.edu
Tian Wang
Clinical Immunology
Mt. Sinai School of Medicine
Tel:(212)-824-7466
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web