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PREAMBLE:
In the wavelength continuum for excitation of fluorochromes, 488nm is the
Everest.
In the quest for extra colours, we can add tandems for 488-excitation, or
we can add light sources which move to higher or to lower wavelengths of
excitation. But which way is better?
Is the He-Ne path of higher wavelength excitation superior to the He-Cd (or
Hg arc) path of shorter wavelength excitation?
The weight of activity definitely vindicates 488 tandems and He-Ne, but why
not UV conjugates like AMCA and tandems for them? Why not viloet-excited
conjugates? Was not the choice driven by what was easy at that time, and
should we not now review it?
THE QUESTION I AM PUTTING: IS CONJUGATE-SPACE SYMMETRIC, OR IS IT BETTER
RED THAN DEAD-violet,..-indigo,...blue??
Have we given UV and violet a fair go in the antibody-fluorochrome
conjugate challenge?
What are the fluorochromes which are suitable conjugates for monoclonal
antibodies, and which excite in the violet or the UV?
Can you give me your tuppence-worth on this?
....still wondering,... Bob
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web