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Ray
On Thu, 1 Feb 1996, Weaverli wrote:
> Help, anyone!
>
> One of my flow "customers" is growing a rat mammary adenocarcinoma
> cell line, MAT B III (which appears in ATCC's repository as CRL 1666)
> for in vitro studies. The cells grow as a static suspension, but in
> aggregates of about 5-10 cells, sometimes more. The problem with
> these cells is not initially dispersing the aggregates, but having
> them remain dispersed. The group has tried an assortment of agents or
> combinations thereof, including trypsin, trypsin-EDTA, EDTA,
> collagenase, DNAse, and hyaluronidase (followed by appropriate
> inhibitors or washing steps to remove enzyme activity.) The
> aggregates disperse, but then re-aggregate, even when placed on ice.
> This results in my turning away whole experiments consisting of clumps
> of very healthy, intact cell aggregates (100% of the cells
> re-aggregate...not a single cell can be found). Mechanical disruption
> produces no single cells.
>
> Is or has ANYONE worked with MAT B III? If so, have you had any
> success with keeping them dispersed for flow analysis? Is it time to
> turn to microscopy/image analysis, or throw in the towel and find
> another cell line? Your comments are appreciated!
>
> Linda Weaver
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
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Phone: (765)-494-0757;
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