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I would like to post a question resulting out of the discussion on this
mailing list about background of flow cytometric analyses held in
October/November 95, I think.
Many, partially contradicting, opinions have been expressed by fellow
"flowers" about the most appropriate and scientific method of background
measurement and subtraction. My knowledge about flow cytometry is far too
little for an accurate assesment of one "standard" or most
respected/accepted method dealing with this issue out of your postings.
Therefore I would like to ask, if there is any consensus or most accepted
method in displaying/calculating e.g. positive cells regarding the problems
with the background or isotypic control. This "consensus" seems very
important to me in regard to straight comparison of publications displaying
flow cytometric analyses and findings.
Any replys are welcomed.
Best regards,
^-^-^-^-^-^-^-^-^-^ KMT-Labor ^-^-^-^ Duesseldorf ^-^-^-^ Germany
^-^-^-^-^-^-^-^-^-^-^
Andreas Christaras
Heinrich-Heine University Duesseldorf, Germany University's
Children Hospital
Laboratory of experimental hematology and stem cell transplantation
(KMT-Labor)
Building 14.82 (Gebauede 14.82) Moorenstrasse 5
40225 Duesseldorf GERMANY
^-^-^-^-^-^-^-^-^-^ B L O O D I S O U R O B S E S S I O N
-^-^-^-^-^-^-^-^-^-^-^
Voice: +49-211-81-16103 Fax: +49-211-81-16206 E-mail:
christar@uni-dueseldorf.de
Notice: due to unfortunately lacking direct internet access, replying to
incoming e-mails may take a couple of days. Your patience in
this issue is appreciated.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web