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I am worried about the appropriate gates to use when analyzing
the frequency of apoptosis by propidium iodide or similar methods. The
sub G0 events are considered to represent apoptotic cells. But is there
any data showing that each such event represents a single cell? Don't
cells (nuclei) break into multiple small fragments? If so, doesn't
counting each of these fragments as a single cell lead to an
overestimation of the actual number of cells undergoing apoptosis?
Any help would be much appreciated.
Henry H. Wortis
Department of Pathology
Tufts University School of Medicine
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web