Reticulated platelets
SCHORER.ANNA@minneapolis.va.gov
07 Dec 95 15:46 CDT
I'm a clinical Hematologist with access to a BD FacScan that is used
for lots of immunophenotyping studies. I'm interested in developing
a reticulated platelet assay on this machine. However, I have some
questions about determining "positive"
v. "negative" in this system. So far, we've run one sample (my lab
tech) with the BD reticstain reagent. We divided her blood into PRP
and whole blood and ran stained & unstained specimens. It didn't seem
to hard to find the RBC and platelets
using forward & side scatter. Does anyone have any suggestions about
finding the proper cut off for "reticulated" red cells & platelets?
[Sorry to ask such basic questions, but I gotta know...]
Thanks in advance for any help.
Anna Schorer, M.D.
schor007@maroon.tc.umn.edu (OR) SCHORER.ANNA@MINNEAPOLIS.VA.GOV
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
