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I don't know about quenching, but phenol red certainly does fluoresce in
all channels on a FACScan.
At the very least it gives high backgrounds, sometimes makes the readings
completely useless.
Avoid it if possible!
Cheers, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web