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YES, I have had good results using lasers that way round. The deviation of
the chromosome peaks from the diagonal is less pronounced but apart from
that the profiles look almost as good. The only time I used the 458 as the
primary laser was using a FACStar Plus with spacial and temporal separation
of signals.
Good luck
Simon
Simon Monard
FACS Lab Manager
Aaron Diamond AIDS Research Center
455 1st Avenue
New York
NY 10016
ph (212) 340 3741
FAX (212) 725 1126
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web