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The critical issue in inducing apoptosis of S phase cells is that the
cells have to move through S phase rapidly. If cultures are somewhat
subconfluent (e.g. at densities over 6 x 105E) the rate of DNA
replication drops and the S phase become more resistant to
camtpothecin.
By the way, if one is using the nuclease treated cells, it may be
better to use micrococcal nuclease, which, similar to the
apoptosis-associated nuclease(s) has preference to the
internucleosomal (linker) DNA sections, rather than DNase.
Zbigniew Darzynkiewicz
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web