Re: fixing and storing cells
T. Scott Thurmond (thurmons@ehsct7.envmed.rochester.edu)
Thu, 13 Jun 1996 06:45:28 -0400
Ruedi Braun wrote:
>
> Does anyone have experience with the fixation of cells after staining
> with FITC or PE?
> I am using 1% paraformaldehyde in PBS and analyze the cells within 12
> hours after fixation.
> We are planing to do some experiments with lavages on humans at high
> altitude and I won't be able to get to a FACS for at least 5 days. Is
> there a possiblity to fix stained cells for such a long time?
> Many thanks for any help in this regard.
> Ruedi
Ruedi,
You should have no analysis problems for cells fixed with 1%
paraformaldehyde for a period of up to 2 weeks, as long as their kept
at 4C. We've been analyzing paraformaldehyde-fixed mouse bone marrow
cells over that period with no apparent loss of signal.
Scott
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
