Re: Identification of Eosinophils in Flow Cytometry
R Barclay (Robin_Barclay@compuserve.com)
Tue, 17 Jun 1997 18:58:55 -0400
Eosinophils are readily identifiable in most leucocyte preps. They run as
a discrete subpopulation (on a BD FACSort) in a forward-scatter (X) versus
side-scatter plot (Y), above the neutrophils and maybe just slightly behind
them (don't turn the side-scatter gain too high, or you'll lose them out
the top of your plot). Also, if you draw a gate around them and look at
this in any fluorescence channel you'll find them to be more
autofluorescent than other leucocytes.
Robin Barclay
BTS Edinburgh
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
