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As far as I know you can only use either FL4 A+W OR FL4H, you can't get FL4
H+W+A on a FACScalibur 4 Color machine.
Neither can you use FL1,2,3 A+W both (only FL1,2,3, A, not W) if you
activate FL4H.
I think it's probably because the 4 Color option was designed as an upgrade
and had to fit into existing FACSsort circuitry... I don't know the exact
technical details (maybe somebody from BDIS could explain them...), but
your are not missing something neither did you find a bug in the software,
things are designed like this... but maybe if we make some noise they'll
realise that this is a badly missing feature and change it in a future
version.
Hope that helps...
Cheers, Matthias
At 10:37 -0400 16/06/97, Frederic Preffer wrote:
>With doublet discrimination option 'on', on FL4, using CellQuest software,
>is it possible to get FL4 height? We cannot and are only getting FL4 width
>and area. We would like to collect FL1,2, & 3 along with all three of these
>FL4 parameters. Is this a 'bug' or missetting in our software, or something
>it is not designed to do?
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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