4-color flow
Darlene Steele-Norwood (steelen@fhs.csu.McMaster.CA)
Mon, 16 Jun 1997 11:57:56 -0400 (EDT)
I am having some problems in setting up the FacStar+ for 4-color flow
using FITC/PE/Tricolor/APC with argon and HeNe lasers and a 650LP
filter. We have been having considerable trouble detecting a Tricolor
signal, although a good signal can be detected using the same sample on
our FacScan. We have used the same antibody in biotinylated form with a
PE/Cychrome secondary (also on the FacStar+) with good results, but this
does not solve our problem as we need to use the biotin for the SA-APC
combination. Has anyone else experienced this problem using the
Tricolor? Any advice on instrument set-up or compensation would be
greatly appreciated.
Darlene Steele-Norwood
McMaster University
Department of Medicine
Hamilton, Ontario
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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, EMAIL
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