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I have a Coulter EPICS ELITE ESP with a 544nm Melles Griot 2mW green laser.
I have not tried Texas Red with this laser, but I have run rhodamine
(TRITC), PE, PI, 7AAD and it works very well. 2mW is sufficient for
immunofluorescense, with the right fluorochrome. According to the
litterature and other users this laser is also a good exitation source for
PE-Cy5, PE-Cy5.5, PE-Cy7, Cy3, Texas Red, LDS751, Po-Pro3, PoPo-3,
PyroninY, DiICn(3), DiTBACn(3), SNARF, SNAFL, DiI and PKH26. Another aspect
of using 544nm laser is that autofluorescense from mammalian cells is much
lower compared to 488nm exitation. This results in higher sensitivity of
immunofluorescense measurements. CVs using this laser are as good as 488nm.
The cost of this 544 air-cooled HeNe laser is low - another good argument
for having this installed.
I think Uniphase also have a low-powered 544nm laser.
Good luck.
Regards,
Kjartan Egeberg
Inst. of Canser Research and Mol. Biol.
Med. Tech. Center
Trondheim
Norway
E.mail: kjartan.egeberg@medisin.ntnu.no
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web