Re: Excitation of Rhodamine...
Howard Shapiro (hms@shapirolab.com)
Wed, 21 May 1997 21:38:35 -0400 (EDT)
>What wavelength(s) excite rhodamine?
>
>From the reference to -dull and -bright cells, I assume you're interested in
rhodamine 123, which is best excited at 488 nm (its absorption maximum is at
485 nm). There are a lot of rhodamine dyes, and they usually are identified
with supplementary letters (e.g., rhodamine B, absorption about 550 nm) or
numbers (e.g., rhodamine 101, the chromophore of Texas red, absorption about
590 nm) or with the relevant chemical substituents added to the name (e.g.,
tetramethylrhodamine, usually conjugated as TRITC, absorption about 550 nm).
None of the three rhodamine dyes just named excite enough at 488 to be
useful; rhodamine 123 is about the only commonly used rhodamine dye with an
absorption wavelength in that neighborhood.
-Howard
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