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I have recently started to look at oxidative burst as a means of
assesing Phagocytosis.
My problem is that when I add the OxyBURST-Green reagent at the
concentrations suggested by molecular probes the cell population moves
dramatically to the origin on Forward and Side scatter. This would
indicate to me that the cells ( this is a tissue culture line) are quite
unhappy. Under the scope there seem to be very few intact cells. (and
unsuprisingly alot of debrie).
Does anyone out there (not on the Heavens Gate tour) have any succesful
experiences with these reagents, and would you be willing to communicate
with me?
Thanks For your Time
Pb
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web