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Does anyone have a protocol to do FCM on isolated nuclei from mammalian
cells? the protocol would be something like, lyse cell membrane, promptly
fix with paraformaldehyde, wash, stain, and sort. We need to stain for a
nuclear protein in the absence of surrounding cytoplasm. Has anyone ever
tried this?
Sincerely,
Don Walker
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web