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We use CD14 as our monocytic marker and it does not seem to be
affected by the stimulation although we have not specifically stained the
cells pre and post stimulation. We had a monocyte product from apheresis
and the staining was as we would have expected. We did see a difference in
IL-12 production depending on the stimulation that the cells received.
Good luck! Stacie
Stacie Anderson
Laboratory of Gene Transfer, Hematopoeisis Section
NCHGR, NIH
Room 2C12
49 Convent Drive
Bethesda, MD 20892-4442
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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