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I have some questions concerning cleaning and maintaining a BD FACScan.
Unfortunately, changing the technicians maintaining and sometimes repairing
the FACScan obviously implies changes in cleaning and maintenance
recommendations and regulations. Initially, following procedure for
cleaning the hydraulic system and measurement capillary on a regular basis
was recommended:
A falcon tube with 0.1% solution of sodium hypochlorite was prepared and
then run over 10-15 minutes with flow control system set to "high flow" and
function switch set to "measurement". Subsequently a tube filled with
destilled water was run over 10-15 minutes (same settings as sodium
hypochlorite solution). This recommendation was exactly the one I found in
the German version of FACScan user's manual by BD.
Later, our lab was recommended to replace the sodium hypochlorite solution
with 0,1% 7 flow or 7X solution and to follow the time limits and settings
as mentioned before for sodium hypochlorite based cleaning.
Last year, after several incidents of hydraulic system block by cell
detritus, recommendation for cleaning was again changed to 0.1% sodium
hypochlorite solution (no change on instrument settings).
Now, as happened yesterday, cleaning recommendation was changed again to
7-flow or 7-X in a 0.1% solution.
If any of you have some idea, information, or experiences indicating the
right and correct (and even the best functioning) method of cleaning the
FACScan, please drop a note. Any incoming suggestions or comments are
appreciated.
Thanks in advance,
Andreas
<><><><><><><><><><><><><><><><><><><><><><KKK KKK MMM MMMM TTTTTTT
Andreas Christaras KKK KKK MMMM MMMMM TTTTTTT
Heinrich-Heine University Duesseldorf KKK KKK MMMMM MMMMMM TTT
Children's Hospital KKKKKK MMM MMM MMM MMM TTT
Dept of pediatric hematology & oncology KKKKK MMM MMM MMM MMM TTT
Lab of experimental hematology (KMT-Labor) KKKK MMM MMMMM MMM TTT
Geb. 14.82 (Bldg.) KKKKK MMM MMM MMM TTT
Moorenstrasse 5 - 40225 Duesseldorf KKK KKK MMM M MMM TTT
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FON : +49-211-81-16103
FAX : +49-211-81-16206 or -16191 BLOOD IS OUR O B S E S S I O N
EMAIL: christar@uni-duesseldorf.de
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web