 |
 |
 |
 |
 |
The Rainbow Calibration Particles from Spherotech (available
through Pharmingen, 1-800-848-6227) have worked well for us for
years. They have a dye mix in them which excites at UV, 488nm,
and dye laser emmission. Using standard Texas Red and APC
collection optics we regularly measusre cv's of less than 7%
with 598 nm dye laser excitation. The intensity in these
channels is approximately two log decades above the background
for unstained lymphoid cells. With 488nm excitation signal
intensities are similar to the dye laser measurements with
better cv's.
-Marty Bigos
Stanford Shared FACS Facility
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web