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It would appear so. Most references to APC either use 633nm (HeNe
laser), 647nm (Kr laser), or a Rhodamine 6G dye laser (tuned to
>590nm). The dye laser is the only laser that will simultaneously
excite TxRed and APC on a commercial sorter, however. At least until
the laser manufacturers can build an "orange" HeNe that puts out more
than 2mW power; they give you strange replies when you ask for 100mW at
594nm. :)
As for laser selection for a new sorter, I think everyone would have a
different opinion. If you can live without TxRed and APC
simultaneously, you can live without a dye laser (and pink floors). :)
/\/\/\_ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu
\ \ \ Analytical Cytometry, Karmanos Cancer Institute and
\_^_/ Immunology & Microbiology, Wayne State University, Detroit, MI
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web