RE: Sorting Sheath Fluid
kukuruga%kasle1.dnet.wayne.edu@rocdec.roc.wayne.edu
Fri, 12 Apr 1996 20:30:26 -0400
Two quick notes....1) If you actually mean "analyze" where you said (typed)
"sort", then HemaLine is ok...if you do mean "sort" -- stop!! CHeck the
formula on HemaLine. I believe it has a mold inhibitor (thymol or some
such) which will most likely kill (or at least mess up!) any cells you may
want to sort viably. 2) What to use? Easiest answer is sterile calcium/
magnesium free PBS. Has several advantages -- isotonic, ions for the
sorter, and it's buffered (good for pH-sensitive stuff like FITC, as
someone recently pointed out to me, after 20 years in the biz).
MAK.
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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