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I want to add my support to Vince Shankey's comments and suggestions
concerning the role that ISAC should play in the use of flow cytometry for
studying apoptosis. I am frequently confronted by people who want to look
for apoptosis by flow and as an example show me a publication using
propidium iodide and looking for a hypodiploid "peak" ( or I must say
"region" because, unfortunately, there is really no peak that is detectable
in quite a few published histograms, even using log scale for analysis!).
The response I get when I point this out is, "Well, they got it
published!"
I would be very interested in topics dealing with apoptosis in human primary
cells and detection of apoptosis ex-vivo (tumours either in human or animal
models).
I will be at Rimini and would be interested in participating in an informal
side gathering on this subject if it can be organized.
Regards,
Kathie Woods-Cook
Pharma Research
Ciba Geigy Ltd.
Basle, Switzerland
email: Kathie.Woods-Cook@chbs.mhs.ciba.com
fax: ***41-61-696-33-89
voice: ***41-61-696-34-30
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web