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There is, in addition, the added complication if using directly
conjugated antibodies. For the isotype control to be valid, it would
also have to match the staining antibody with respect to its F/P ratio.
And accurate determination of F/P ratios for antibodies used in flow
cytometry is a huge can of worms.....
Alice Givan
Englert Cell Analysis Laboratory Dartmouth Medical School
Lebanon, New Hampshire
USA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web