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Eosinophils express CD49d (VLA-4) on their surface whereas neutrophils do
not. By gating on the granulocyte population (on a FS vs. SS plot), and
CD49d expression you can identify eosinophils.
The other method I've used to identify eosinophils is by polarised light
scatter. Eosinophils depolarise light to a greater extent than other white
cells so they can be distinguished quite easily. Since the laser provides
the polarized light source all you need is a polarizing filter. I've used
this method on our EPICS XL. A reference for this method is:
B.G. de Grooth, L.W.M.M. Terstappen, G.J. Puppels, and J. Greve.
Light - Scattering Polarization Measurements as a New Parameter in Flow
Cytometry. Cytometry 8:539-544 (1987).
- Maria Daly.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web