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Specifically, I'm using a FACStar Plus. The question comes up
because in a direct conjugate, low signal/noise situation, the best S/N
occurs at low laser power (autofluorescence requires higher power than FITC
or tetramethyl rhodamine for half-maximal excitation). Therefore, I end up
with low emission intensities, particularly in the red. I want linear (not
log) acquisition, so the red PMT voltage will need to be high if I keep the
laser power at minimum.
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web