fluorescence intensity from large cells/estimating surface

mohedley@spiff.pmh.toronto.on.ca
Tue, 18 Apr 1995 09:21:37 -0500

We are very interested in the problem of obtaining the concentration of
glutathione in cells, since this is llikely to give a better reflection of drug
resistance than whole cell GSH content We are attempting to approximate
volume by obtaining diameter from time of flight, using an Elite. This is a
three way interaction with Kit Snow at Coulter, and David Galbraith at
University of Arizona, Tuxson. We are considering conversion of diameter
to volume with hardware, or off line using a program like Winlist.

It seems to me that the ability to derive volume in actual SI units might be
of widespread use in flow, particularly dealing with biochemical reactions
where the reaction rate is determined by concentration of reactant rather
than total amount of substance. Aesthetically, I also like the idea of flow
cytometers giving results in the same units of measurement as the
hematology instruments; you can never have too much quality control in
this game!
I would be interested to make contact with any others working in this field.

David Hedley
Ontario Cancer Institute/Princess Margaret Hospital
Toronto


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu