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Is the hinged dichroic holder you are using recycled from an old BD
instrument? If so, we tried that too, and failed to get any purple signal
out. The Plus comes with a dichroic holder that is stationary and I rigged
(with cardboard and double backed tape) a way to install my 440 LP onto that
holder. Hey, it works!
We use the Bright Blue Fluorescebrite (sp?) beads from Flow Cytometry
Standards Corp. to align the UV laser and get great signal into both the 485
and 405 PMTs.
Lisa Johnson
UT Southwestern
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web